| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168709 |
| Chromosome: | chromosome 1 |
| Location: | 7489635 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053850 | (1 of 2) K10143 - E3 ubiquitin-protein ligase RFWD2 (RFWD2, COP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCATGCGTTGCACCACCGCTAGCAGTC |
| Internal bar code: | GGGGTGTCGACTGCGCCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 73.0674 |
| LEAP-Seq n confirming: | 4140 |
| LEAP-Seq n nonconfirming: | 1526 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCTTCTCATTGCGGTGG |
| Suggested primer 2: | CTGTCGTCTCTGTCGTTGGA |