| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168930 |
| Chromosome: | chromosome 9 |
| Location: | 3493092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389689 | ASD1,ASSD1 | Aspartate semialdehyde dehydrogenase; (1 of 1) 1.2.1.11 - Aspartate-semialdehyde dehydrogenase / L-aspartate-beta-semialdehyde dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGGGACAGTCCGACGAGGTTATGGGG |
| Internal bar code: | GAGTATCTCCGAAGCGAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1178 |
| LEAP-Seq percent confirming: | 86.2216 |
| LEAP-Seq n confirming: | 1821 |
| LEAP-Seq n nonconfirming: | 291 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTTCCTTTTGACGTGC |
| Suggested primer 2: | GCTGAGCTATGCCTCTGGTC |