| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168930 |
| Chromosome: | chromosome 9 |
| Location: | 3493097 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389689 | ASD1,ASSD1 | Aspartate semialdehyde dehydrogenase; (1 of 1) 1.2.1.11 - Aspartate-semialdehyde dehydrogenase / L-aspartate-beta-semialdehyde dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGCATCCATAACCTCGCATTCCCCCCA |
| Internal bar code: | CTCTTTTCACCTTTAGATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1250 |
| LEAP-Seq percent confirming: | 99.7253 |
| LEAP-Seq n confirming: | 1452 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTTCCTTTTGACGTGC |
| Suggested primer 2: | GCTGAGCTATGCCTCTGGTC |