Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.169214 |
Chromosome: | chromosome_7 |
Location: | 1237774 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g321400 | FAP113 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGTGGCGCCGTTTGGCATGAGTATTGAGA |
Internal bar code: | ATTCGGGGTCAGTCAACGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 0 |
LEAP-Seq percent confirming: | 99.7859 |
LEAP-Seq n confirming: | 932 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTTCCAGAAAGCACACAT |
Suggested primer 2: | GTGTCGTTACGGCCTTGATT |