| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169324 |
| Chromosome: | chromosome 3 |
| Location: | 2306850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158250 | SCY2,SECY2 | (1 of 1) PTHR10906:SF2 - PREPROTEIN TRANSLOCASE SUBUNIT SCY2, CHLOROPLASTIC; Preprotein translocase secY subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAAGTAAATATGGTTGTACGGGGAAGGC |
| Internal bar code: | TTTTTTGTCGTATCCCCAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1048 |
| LEAP-Seq percent confirming: | 96.545 |
| LEAP-Seq n confirming: | 4471 |
| LEAP-Seq n nonconfirming: | 160 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACATCCTCCGTGCTGAT |
| Suggested primer 2: | CCTGCTGCACAGTGTTGAGT |