| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169620 |
| Chromosome: | chromosome 13 |
| Location: | 1879821 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575650 | (1 of 2) PTHR24056//PTHR24056:SF107 - CELL DIVISION PROTEIN KINASE // CYCLIN-DEPENDENT KINASE 11A-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGTCGGATGGGTGGGTAGTCGTCCCG |
| Internal bar code: | CCCAGCAGTACTCCTCGGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 99.8693 |
| LEAP-Seq n confirming: | 1528 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATGTGGTCACTCTGTGG |
| Suggested primer 2: | CTCCTCACTTTCGAACCAGC |