Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.169645 |
Chromosome: | chromosome_7 |
Location: | 4819300 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g345900 | DCL3 | Dicer-like protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCATGTGAGATTGGATGCTGTTGTGCGCAT |
Internal bar code: | TTTTGTAATTTAGGCCGTGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 829 |
LEAP-Seq percent confirming: | 92.2055 |
LEAP-Seq n confirming: | 156079 |
LEAP-Seq n nonconfirming: | 13194 |
LEAP-Seq n unique pos: | 254 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAAATTTTCCGTTGCATCA |
Suggested primer 2: | ACCAGAATCACCAGCAGGAC |