Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.169645 |
Chromosome: | chromosome_7 |
Location: | 4819303 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g345900 | DCL3 | Dicer-like protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAAGTTCTGGAAAGCACCGCAACGGCTGT |
Internal bar code: | GCCGTTCGCTTCACCGTGGCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 454 |
LEAP-Seq percent confirming: | 16.6986 |
LEAP-Seq n confirming: | 2004 |
LEAP-Seq n nonconfirming: | 9997 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCAGAATCACCAGCAGGAC |
Suggested primer 2: | TCAAATTTTCCGTTGCATCA |