Insertion junction: LMJ.RY0402.170311_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.170311
Chromosome:chromosome 13
Location:2222567
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGCTCTGCACTGCCGCCACCTCCTCCA
Internal bar code:CGCCTGCTATATTTTTCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:24
LEAP-Seq percent confirming:99.7519
LEAP-Seq n confirming:402
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTACAATGTCGCAAGGAGCA
Suggested primer 2:GATGAGGGCCTGTGTGTTTT