| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170533 |
| Chromosome: | chromosome 9 |
| Location: | 2885668 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388372 | RAT2,OPR39 | tscA RNA stabilization factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTTATTCAAGGCAGTACGACATGACCG |
| Internal bar code: | TCAGGTATTCTTATTTTCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACTGATTGAGCGGCTAC |
| Suggested primer 2: | GCTTGCTAAACAGGTCAGGC |