| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.170786 |
| Chromosome: | chromosome_9 |
| Location: | 567499 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g403800 | DYHB,ROC30,ROC54,ODA4 | Flagellar outer arm dynein heavy chain beta, involved in circadi | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACCTCGCGGTCCAGCAGGCCCTCAAAACT |
| Internal bar code: | AACAGATCCTGGAATCAAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.4534 |
| LEAP-Seq n confirming: | 4367 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGACGTGATGGATGTGG |
| Suggested primer 2: | ATCTCAGCGCACTTCCAGTT |