| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171437 |
| Chromosome: | chromosome_1 |
| Location: | 3900298 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025400 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAGGACAACCCCACGCCGGTGGAGTTCCC |
| Internal bar code: | GAGTGGGTCGGACAAGTTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 99.7524 |
| LEAP-Seq n confirming: | 2417 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTTGATCACGATGGGCT |
| Suggested primer 2: | CTTCAAGGTGTTCACGCTCA |