| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171945 |
| Chromosome: | chromosome_1 |
| Location: | 3899372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025400 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCGCATACTTGCCTGTGGGACGGCAAGCA |
| Internal bar code: | AGTGCCTCGGGGGCAACGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 96.8539 |
| LEAP-Seq n confirming: | 14038 |
| LEAP-Seq n nonconfirming: | 456 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTCGTTGTTGATGGGGT |
| Suggested primer 2: | GTGGAGGACGTCATTGTGTG |