| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171945 |
| Chromosome: | chromosome_1 |
| Location: | 3899372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025400 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCGTACAACTTCAGCATGAAGCCGCTGTC |
| Internal bar code: | GGGTGTGATGGTCTAACAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 97.3388 |
| LEAP-Seq n confirming: | 3109 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTCGTTGTTGATGGGGT |
| Suggested primer 2: | GTGGAGGACGTCATTGTGTG |