| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172076 |
| Chromosome: | chromosome 3 |
| Location: | 6093167 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGAGACACCCTCCAGGGGCTGGCTCT |
| Internal bar code: | GCATTAGTAAGGGGGTAGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 99.8377 |
| LEAP-Seq n confirming: | 1230 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTGTCCTCGCTCTCATT |
| Suggested primer 2: | TCACAACGTAGCTGAGGACG |