Insertion junction: LMJ.RY0402.172449_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.172449
Chromosome:chromosome 1
Location:498761
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGCATCAAATTTTGGCACGTGGTTCGGC
Internal bar code:ATTGCGGGTCCCGATCGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:98.6111
LEAP-Seq n confirming:142
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATCACGCCTGTTTGATTTG
Suggested primer 2:ATAAACCCGGGCCTAACAAC