Insertion junction: LMJ.RY0402.172652_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.172652
Chromosome:chromosome 10
Location:3105420
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g441650 (1 of 1) PF06650//PF16908 - SHR-binding domain of vacuolar-sorting associated protein 13 (SHR-BD) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTCTGCCACGCCTCGTCCTGGTAGACG
Internal bar code:TGTACCCCACCCGTTTTGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:851
LEAP-Seq percent confirming:99.7723
LEAP-Seq n confirming:6572
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGTTGATCAGCTGGAAG
Suggested primer 2:GGAGCTTTTCAACTTCACGC