Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.172652 |
Chromosome: | chromosome 10 |
Location: | 3105420 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g441650 | (1 of 1) PF06650//PF16908 - SHR-binding domain of vacuolar-sorting associated protein 13 (SHR-BD) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCTGCCACGCCTCGTCCTGGTAGACG |
Internal bar code: | TGTACCCCACCCGTTTTGAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 851 |
LEAP-Seq percent confirming: | 99.7723 |
LEAP-Seq n confirming: | 6572 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGACGTTGATCAGCTGGAAG |
Suggested primer 2: | GGAGCTTTTCAACTTCACGC |