Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.172872 |
Chromosome: | chromosome_10 |
Location: | 3199043 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g442600 | XUV5,UAPA2 | Xanthine/uracil/vitamin C permease-like | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATGCGTGCTGAACATCCTACACCACCAGT |
Internal bar code: | TAGACACGGGTCGCTAGCGATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 539 |
LEAP-Seq percent confirming: | 99.7283 |
LEAP-Seq n confirming: | 1101 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCACCCTACGGGACTTTAC |
Suggested primer 2: | GTACTGGCCTGGGTAAACGA |