| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172902 |
| Chromosome: | chromosome 12 |
| Location: | 61512 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483700 | RBP40,RB38 | Chloroplast-targeted RNA-binding protein; (1 of 2) PTHR10302//PTHR10302:SF8 - SINGLE-STRANDED DNA-BINDING PROTEIN // PROTEIN OSB2, CHLOROPLASTIC-RELATED | 3'UTR|outside_mRNA |
| Cre12.g483720 | (1 of 2) PTHR10302//PTHR10302:SF8 - SINGLE-STRANDED DNA-BINDING PROTEIN // PROTEIN OSB2, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCCCCCCCGCTGGTCGTAGTCGAGCGC |
| Internal bar code: | TCGGCTCGCCGTCGCACTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAGTGCAAGTAGAGGTG |
| Suggested primer 2: | TGCGACATACCTTGCTCTTG |