| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173195 |
| Chromosome: | chromosome 5 |
| Location: | 1924967 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241634 | HAV2 | Histone H2A variant | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCCTCCTTCCCCTCCTTGCCCGTCTTC |
| Internal bar code: | GCAGGCACGTCATCCGGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 90.7244 |
| LEAP-Seq n confirming: | 72184 |
| LEAP-Seq n nonconfirming: | 7380 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTGCACCATCAAATACT |
| Suggested primer 2: | CCTTCATGTGTGACCCTCCT |