| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173287 |
| Chromosome: | chromosome 10 |
| Location: | 4609699 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452800 | LCIB1,PMP1,LCIB | Low-CO2-inducible protein B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAACGAGGAGCCGAAGATGGCCTCAATC |
| Internal bar code: | GGTCTTCGAGTCGGACCTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 98.7013 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCGACTCTGCTTTGTG |
| Suggested primer 2: | GGTGCTGGTGGAGTTAGGAA |