| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173314 |
| Chromosome: | chromosome 3 |
| Location: | 4721066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177950 | (1 of 1) PTHR32093:SF10 - MUCIN 14A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGCCTCTTCTCAGCTCGAACTGATGT |
| Internal bar code: | ACTCAGTTTGGGGCTTCTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 79.0338 |
| LEAP-Seq n confirming: | 818 |
| LEAP-Seq n nonconfirming: | 217 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACCTTGCAAAGCAGAG |
| Suggested primer 2: | AGCTCTGCCTCTCGCAGTAG |