Insertion junction: LMJ.RY0402.173971_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173971
Chromosome:chromosome 13
Location:2226677
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACAATCCCCGCCTCCATCCACACACACA
Internal bar code:CACCCCGCAACCCTGGCCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:366
LEAP-Seq percent confirming:24.5176
LEAP-Seq n confirming:775
LEAP-Seq n nonconfirming:2386
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCTGAATGGAGGTGAAGG
Suggested primer 2:GATTTCGTGGTGGAAGAGGA