| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174140 |
| Chromosome: | chromosome 16 |
| Location: | 6069291 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676150 | MSD3 | (1 of 1) PTHR11404//PTHR11404:SF22 - SUPEROXIDE DISMUTASE 2 // 37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL; Mn superoxide dismutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGAACAAGACGACGAAGACGGCGCGAT |
| Internal bar code: | GCGTCCGGGCGGCAGTCCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.4466 |
| LEAP-Seq n confirming: | 5930 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTACACCACTGACAAGA |
| Suggested primer 2: | CAGTGGTTGGTACTCCCCTG |