Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.174255 |
Chromosome: | chromosome_12 |
Location: | 3050228 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g500550 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGACGGCAGGCTGGACAAACTGCTCAAGCA |
Internal bar code: | GAGAATGGTAGTCGATCGCTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 308 |
LEAP-Seq percent confirming: | 57.6998 |
LEAP-Seq n confirming: | 1776 |
LEAP-Seq n nonconfirming: | 1302 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTTTGTTTCCCCAGCTTT |
Suggested primer 2: | CAACAACATTGGTGGTCGTC |