Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.174351 |
Chromosome: | chromosome_1 |
Location: | 849992 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g004350 | ALIX,HLM1,ROC77 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGCTTGGGGCAGTCAGAAGACAGACATA |
Internal bar code: | GGCTTCGCACCTGGGCGAGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 791 |
LEAP-Seq percent confirming: | 99.6763 |
LEAP-Seq n confirming: | 6775 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTGAAATTGGTGTGTCAG |
Suggested primer 2: | CATGGCTTGGCTGATGTATG |