| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174381 |
| Chromosome: | chromosome 16 |
| Location: | 6069213 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676150 | MSD3 | (1 of 1) PTHR11404//PTHR11404:SF22 - SUPEROXIDE DISMUTASE 2 // 37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL; Mn superoxide dismutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCATTCCCTGTGTTTCCGGTAGCGCACA |
| Internal bar code: | TGAGAGGGAGTGTCACCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 99.8998 |
| LEAP-Seq n confirming: | 1995 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTACACCACTGACAAGA |
| Suggested primer 2: | CAGTGGTTGGTACTCCCCTG |