| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174518 |
| Chromosome: | chromosome_16 |
| Location: | 1159926 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g650450 | FAP219 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCGCCGCGGCTTGCGGGTCCATGCTACCA |
| Internal bar code: | CCCGCCACTGTTCGAGAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 95.7797 |
| LEAP-Seq n confirming: | 13912 |
| LEAP-Seq n nonconfirming: | 613 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGGTGGTGTAGCCCTG |
| Suggested primer 2: | CACTCAGCCCACAACTCAGA |