| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174576 |
| Chromosome: | chromosome 12 |
| Location: | 7286577 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557700 | (1 of 1) K01069 - hydroxyacylglutathione hydrolase (E3.1.2.6, gloB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGGGAAAGAGATGGCGTGCGTGTGGTT |
| Internal bar code: | AGTATTGCTTAGCTAAGTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 99.5122 |
| LEAP-Seq n confirming: | 3264 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACTCCTGAAGCACACA |
| Suggested primer 2: | TGCGCTATGTTTTCCTTGTG |