| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174670 |
| Chromosome: | chromosome 7 |
| Location: | 6189211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356250 | CYP741A1,CYP23 | (1 of 1) 1.14.13.173 - 11-oxo-beta-amyrin 30-oxidase / CYP72A154; Cytochrome P450, CYP197 superfamily | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTACTCCCGTTCATCAGGCTTGAGGCAT |
| Internal bar code: | GGTCGGGCCCAGGCGTACAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 98.8299 |
| LEAP-Seq n confirming: | 1098 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGATACCGTGCCTCACC |
| Suggested primer 2: | TGGTACTTGGACCGTGTGAA |