| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176081 |
| Chromosome: | chromosome 12 |
| Location: | 3570979 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513500 | XAB2 | (1 of 1) K12867 - pre-mRNA-splicing factor SYF1 (SYF1, XAB2); Transcription-coupled DNA repair protein, possible splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGACCTGGACGCTGACGCGGACGACGA |
| Internal bar code: | GGTTCCGGGGTAGTCCCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 87.1417 |
| LEAP-Seq n confirming: | 4378 |
| LEAP-Seq n nonconfirming: | 646 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCCAATATGCTGCTCT |
| Suggested primer 2: | CCACATCTGGACAAGGGAGT |