Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.176258 |
Chromosome: | chromosome_17 |
Location: | 3681731 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g726850 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCCAGCAAATACGTGCAAGCTAACACTCT |
Internal bar code: | GAACGGGACGCGAGTGACCTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 508 |
LEAP-Seq percent confirming: | 99.7087 |
LEAP-Seq n confirming: | 4107 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGGTCATCAACATCCACG |
Suggested primer 2: | CTAGCCTCTGCCCATCAGTC |