| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176807 |
| Chromosome: | chromosome 3 |
| Location: | 4558109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176833 | NAP1,NAP,DII5 | (1 of 7) PF00022 - Actin (Actin); Novel actin protein 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGTGATGTGGTAAGGTATAAGGTGCCG |
| Internal bar code: | GAATGTACACGTGGGGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 37.5566 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTAAGGGGTGGTTGATT |
| Suggested primer 2: | TGAGCTTTGGACTACCCCAC |