| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176819 |
| Chromosome: | chromosome_13 |
| Location: | 2132797 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577950 | VPS60 | Subunit of the ESCRT-III complex | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAAGGCGTACTTGGTGGTCGTGTTGGAC |
| Internal bar code: | GCCCACGTGGGCCCACCTAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 945 |
| LEAP-Seq n nonconfirming: | 280 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTACAGCGTGTGACTGC |
| Suggested primer 2: | CGCTGCACTGACTGCTACTC |