| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176901 |
| Chromosome: | chromosome 1 |
| Location: | 1830846 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009900 | CDJ3 | Chloroplast DnaJ-like protein 3; (1 of 2) IPR001080//IPR001623//IPR017896 - 3Fe-4S ferredoxin // DnaJ domain // 4Fe-4S ferredoxin-type, iron-sulphur binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTCCGCTCTCACGTGGCTGTGGTGATG |
| Internal bar code: | GCTGGCTTCCGTTGTCCATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 99.6169 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTAGCCCTGGTTGTGGG |
| Suggested primer 2: | TGCAATTTTAGTGCAGGCAG |