Insertion junction: LMJ.RY0402.176928_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.176928
Chromosome:chromosome 4
Location:3780157
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTAAGTTTCGCGGCACGTACGGCGTGGGT
Internal bar code:AATTCGGTTCTTGTGTCGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:98.2545
LEAP-Seq n confirming:5404
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCTGACTGCGATGATGAC
Suggested primer 2:CTGTCAGGCAGTAGCAGCAG