| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177040 |
| Chromosome: | chromosome 2 |
| Location: | 5886432 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111450 | TEF4 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese); Rhodanese-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCACGTGCTGATGACGCCCGGCCGTAG |
| Internal bar code: | GTGTCGCCCTGACGGTAATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 50.7791 |
| LEAP-Seq n confirming: | 554 |
| LEAP-Seq n nonconfirming: | 537 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAAAGCGACAGCGTCTT |
| Suggested primer 2: | CATCGCTCTGCATGACAGTT |