| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177271 |
| Chromosome: | chromosome 11 |
| Location: | 389668 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467579 | CSC6 | Chlamydomonadale-specific family C protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACGCATTGCTGTCGGTGTGTGACTGC |
| Internal bar code: | TTGTATCTCCGACAAGGATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 99.3655 |
| LEAP-Seq n confirming: | 1566 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAACACGGACAAACCAGC |
| Suggested primer 2: | ACTAAGGGTGACATGCCGTC |