| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177660 |
| Chromosome: | chromosome 16 |
| Location: | 5283011 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682550 | (1 of 1) PF08016//PF08263//PF13855 - Polycystin cation channel (PKD_channel) // Leucine rich repeat N-terminal domain (LRRNT_2) // Leucine rich repeat (LRR_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGAATTCGAGACGGTTTGAGATCTTAC |
| Internal bar code: | GACGGCGCCTCAACGTTTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 71.9665 |
| LEAP-Seq n confirming: | 1204 |
| LEAP-Seq n nonconfirming: | 469 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAATGGAGGTGAAGAAG |
| Suggested primer 2: | GCAGGACGGGAATGTGTACT |