| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178018 |
| Chromosome: | chromosome 8 |
| Location: | 3672879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376700 | MRCE1,MRC1 | (1 of 1) K13917 - mRNA-capping enzyme (RNGTT); mRNA capping enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATAAAACAAGAGAAGCTCTCCCTCCGTC |
| Internal bar code: | GCGTGTAACGGATTCCCACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.8636 |
| LEAP-Seq n confirming: | 732 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGAGCGATCCATGATGT |
| Suggested primer 2: | GTCGTATGCAACCTCCACCT |