| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178610 |
| Chromosome: | chromosome 14 |
| Location: | 494269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611300 | COP3,CHR1,CSOA | (1 of 7) PF01036 - Bacteriorhodopsin-like protein (Bac_rhodopsin); Channelrhodopsin 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTTGTTGATACTGTATTAATCATGCGT |
| Internal bar code: | GAGAGGGGTGACCGCGTGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 98.0018 |
| LEAP-Seq n confirming: | 3335 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGGGCCAGAAACAGACC |
| Suggested primer 2: | GGGGAAGAAGAGGCTACCAC |