Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.178917 |
Chromosome: | chromosome_7 |
Location: | 1244661 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g321400 | FAP113 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGACAAGGGCAAGGGAGGTTTCACCTTC |
Internal bar code: | TACATGCACTGATCGCTTACCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 163 |
LEAP-Seq percent confirming: | 95.6522 |
LEAP-Seq n confirming: | 22 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTGGCGTAGGTGATCTT |
Suggested primer 2: | GCACCTTCCTCAGTCCTCTG |