| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179015 |
| Chromosome: | chromosome 1 |
| Location: | 4053528 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026450 | SRP35,SRS1 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 2) K12890 - splicing factor, arginine/serine-rich 1/9 (SFRS1_9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGGGTCTCTTTAGCGCTGCACTTCGG |
| Internal bar code: | CGTACGTAGAGCCGGCCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 96.2353 |
| LEAP-Seq n confirming: | 3272 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGACCTGAAGGACCACAT |
| Suggested primer 2: | GCACAATGTCACCATTCGTC |