| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179271 |
| Chromosome: | chromosome_3 |
| Location: | 1430975 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g151250 | LANC1,LAN1 | LanC lantibiotic synthetase component C-like protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACCGCACTGGCAACACACCACTCGCCGA |
| Internal bar code: | TGGTCCTCATAGACACGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 96.9212 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCAGTGATTTGTGTTGG |
| Suggested primer 2: | AACACATGAGGAGCTGGGAC |