Insertion junction: LMJ.RY0402.179271_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179271
Chromosome:chromosome 3
Location:1430975
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCGCACTGGCAACACACCACTCGCCGA
Internal bar code:TGGTCCTCATAGACACGGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:631
LEAP-Seq percent confirming:96.9212
LEAP-Seq n confirming:787
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTCAGTGATTTGTGTTGG
Suggested primer 2:AACACATGAGGAGCTGGGAC