Insertion junction: LMJ.RY0402.179271_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.179271
Chromosome:chromosome 3
Location:1431045
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCCTCCTGTGACCCCGGAGGGCTTGGA
Internal bar code:CAATGTCCATGTGTTTTTCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:858
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTCAGTGATTTGTGTTGG
Suggested primer 2:AACACATGAGGAGCTGGGAC