Insertion junction: LMJ.RY0402.179271_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179271
Chromosome:chromosome 10
Location:4436105
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g451600 SRR28,SRR28B (1 of 1) PF00059//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4); Lectin-domain glycosyl hydrolase intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCACCACACGCTGACGTAGGACTGGC
Internal bar code:GGCCCAATCAGTGTAGCCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:980
LEAP-Seq percent confirming:99.7582
LEAP-Seq n confirming:825
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAATGCTGCTGATGTCCTGC
Suggested primer 2:GCGCTGTGTCAACCTGACTA