| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179271 |
| Chromosome: | chromosome_10 |
| Location: | 4436123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g451600 | sense | intron|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGATGAGTATGGTTGTCGCCAAACGGCAA |
| Internal bar code: | TTTGGGACCCGGCCCAGATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 99.5021 |
| LEAP-Seq n confirming: | 1199 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGTGTCAACCTGACTA |
| Suggested primer 2: | TAATGCTGCTGATGTCCTGC |