| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179649 |
| Chromosome: | chromosome 13 |
| Location: | 1106730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569300 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGAGGCGCCATGTCGCGGCCGGACGGC |
| Internal bar code: | TTCGTCACTATTTACAAGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.6587 |
| LEAP-Seq n confirming: | 292 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAAGATGGCTGTTTGAT |
| Suggested primer 2: | TGCCAGCGTACTGCTATGAG |