| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179953 |
| Chromosome: | chromosome 3 |
| Location: | 3884589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171179 | (1 of 226) IPR012337 - Ribonuclease H-like domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCTGTGCAGCGACCAGCACTGTAACG |
| Internal bar code: | GTCGAACGGTTTGGCCCGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 98.3819 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTCTTCTCGCGCTTCAG |
| Suggested primer 2: | GAGTGCGAGTTGTAGGGAGG |