Insertion junction: LMJ.RY0402.180475_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.180475
Chromosome:chromosome 14
Location:3729061
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632050 POB25,RPG1,RPGRIP1 Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGAGAAAGCGGGGGGGGGGGGGGCTGGG
Internal bar code:TTATATTTTTGGGATCGGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:156
LEAP-Seq percent confirming:99.7917
LEAP-Seq n confirming:479
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACTGGATGACCGTGTTG
Suggested primer 2:TCAAGAAGCTGCACAAGGTG